Immunohistochemical Localization of YAP and TAZ in Mouse Molar Tooth Germ

Tooth development is a multi-stage and multi-step process involving fate determination morphogenetic patterning events, epithelial-mesenchymal interactions, cell proliferation, differentiation, migration. Animal model studies have shown that YAP TAZ, effectors of the Hippo pathway, critical function in tooth morphogenesis. However, TAZ has not been well documented its specific roles morphogenesis remain unclear. We used immunohistochemistry to examine localization mouse mandibular first molar germ. ICR embryos on days E12, E14 E18 were produced. Heads from these processed for paraffin embedding prepared immunohistochemistry. Immunostaining showed different development. was localized odontogenic epithelium early stages germ, but almost observed. also positive pre-ameloblasts or inner enamel epithelium, which high columnar cells. On other hand, odontoblasts epithelium. These results demonstrated functional differences between development, suggested proteins only involved hard tissue formation, three-dimensional cusp during